Method development and validation of capillary electrophoresis: A practical aspect

Method development and validation of capillary electrophoresis: A practical aspect

Abstract

The current review illustrates the method development and validation of capillary electrophoresis (CE) from experiences of the author research group. Various examples including drug monitoring, pharmaceutical, and natural product analyses are described. The author emphasizes that no specific CE condition is applicable for all problems. Analytes’ physicochemical properties (e.g., solubility, dissociation constant, polarity, absorptivity, etc.) and their stability are major consideration. Sample matrices (e.g., biological fluid and tissues, foods, medicinal plants, etc.) can usually complicate an analysis and should be accounted for before a method is developed. Consequently, sample pretreatment/preparation procedures have to be carefully optimized as well. Read More

Feature Extraction of a Synchronized Swimmer from Underwater Videos

Feature Extraction of a Synchronized Swimmer from Underwater Videos

Abstract

Vision-based feature extraction of a synchronized swimmer doing figures was done in the hopes of correcting and improving a synchronized swimmer’s ability to do figures. Extracted feature through image processing techniques plays an important role in understanding the synchronized swimmer’s body kinematics and dynamics. In gathering data, two cameras were used to video capture the swimmer’s movements. One was stationed at a calculated distance above the water, and the other camera was placed underwater directly above the other camera. Read More

Skewered Cyano-Bridged Cobalt Naphthalocyanine Polymer: An Electron Transport Engineering

Skewered Cyano-Bridged Cobalt Naphthalocyanine Polymer: An Electron Transport Engineering

Abstract

Naphthalocyanines (Nc) are ideal molecular conductors due to their flat and fully-conjugated structures. Attachment of a central metal (Co) and dicyano (CN) axial ligands to Nc produced CN-bridged cobalt naphthalocyanine, a skewered-type polymer complex. The polymeric species enabled intra-chain electron transport through the CN bridge by which, coupled with the interchain p-p overlaps, produced a multi-dimensional electronic system. Read More

Irreversibility Lines for REBa2Cu3O7-d Crystals

Irreversibility Lines for REBa2Cu3O7-? Crystals

Abstract

The irreversibility lines (ILs) for high quality REBa2Cu3O7-? crystals (RE = Y and Nd) were determined from complex magnetic susceptibility measurements. The effect of twins on the crystals was also investigated by comparing the ILs for the YBa2Cu3O7−? samples with and without twins. The YBa2Cu3O7−? single crystals were grown through the flux method while the intergrowth Nd1/2Y1/2Cu3O7-? crystals were grown using the melt-textured growth technique. Stoichiometric analysis through XRD and EMPA showed a single phase YBa2Cu3O7−? crystal and a dual phase (Nd/Y)Ba2Cu3O7−? composite crystal. Read More

snip20161017_1Establishing Genetic Association Among Selected Members of Citrus Species Through Protein Profiling Obtained from Comparative Electrophoresis

Establishing Genetic Association Among Selected Members of Citrus Species Through Protein Profiling Obtained from Comparative Electrophoresis

Abstract

The use of seed proteins in the evaluation of genetic diversity is cost effective and less time consuming. Water and salt-soluble proteins from the seeds of Citrus species: C reticulata, C. aurantifolia, C grandis, C. sinensis, C. limon, and C. microcarpa were extracted and variations in electrophoretic profiles were determined. Finely grounded seeds were homogenized in 50 mM Tris buffer, pH = 7.0, centrifuged at 14,000 rpm for 10 minutes at 4ºC, and the supernate collected as the water-soluble fraction. The pellet was resuspended in 50mM Tris buffer, pH = 7.0 in 150 mM NaCl, homogenized, then centrifuged at 14,000 rpm for 10 minutes at 4ºC, and the supernate collected as the salt-soluble fraction. Samples were analyzed using SDS PAGE. Read More